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KMID : 0525720140190020122
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Journal of Chitin and Chitosan
2014 Volume.19 No. 2 p.122 ~ p.129
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Synthesis of O-carboxymethyl Chitosan Modified Transferrin and PEI for Efficient Gene Delivery
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Park Sun-Jeong
Byeon Ji-Ye
Park So-Young
Kim Young-Min
Nam Joung-Pyo
Park Seong-Cheol
Park Yung-Hoon
Nah Jae-Woon
Jang Mi-Kyeong
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Abstract
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The polyethyleneimine / O-carboxymethyl chitosan / polyethyleneglycol / transferrin (POPT) copolymer was evaluated as nonviral gene delivery carrier. The structural analysis of POPT copolymer was confirmed by 1-proton nuclear magnetic resonance ( 1 H-NMR). The particle size of - pEGFP-N1/POPT polyplexes was confirmed by dynamic light scattering (DLS). The results of DLS showed that the particle sizes of pEGFP-N1/POPT polyplexes has small size of 130~260 nm. The binding ability with pEGFP-N1, pEGFP-N1 protecting assay against DNase, and pEGFP-N1 release assay through heparin of POPT were evaluated by gel retardation assay. In addition, gene transfection effect of pEGFP-N1/POPT polyplexes was evaluated in fibroblast cell line (L929, without transferrin receptor) and cervical cancer cell line (HeLa-S3, with transferrin receptor) cells. The results of transfection were shown that green fluorescence was cancer cell more expression than normal cell due to receptor mediated endocytosis between transferrin of POPT and transferrin receptor on the cancer surface. Therefore, pEGFP-N1/POPT polyplexes could be a promising gene delivery system.
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KEYWORD
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O-carboxymethyl chitosan, Polyethyleneimine, Transferrin, Gene delivery
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